• Latest
  • Trending
Development and validation of a multiplex real-time RT-PCR test for the screening of tomato (Solanum lycopersicum) for the detection of pepino mosaic virus

Development and validation of a multiplex real-time RT-PCR test for the screening of tomato (Solanum lycopersicum) for the detection of pepino mosaic virus

August 28, 2024
Peruvian Asparagus in Europe: Seizing the Seasonal Window for Export Growth

Peruvian Asparagus in Europe: Seizing the Seasonal Window for Export Growth

June 24, 2025
Brazil’s Fruit Export Paradox: Why the World’s Third-Largest Producer Struggles to Dominate Global Markets

Brazil’s Fruit Export Paradox: Why the World’s Third-Largest Producer Struggles to Dominate Global Markets

June 24, 2025
Price Volatility in Vegetable Markets: Analyzing Trends in Muhana Mandi and Their Impact on Farmers

Price Volatility in Vegetable Markets: Analyzing Trends in Muhana Mandi and Their Impact on Farmers

June 23, 2025
Revolutionizing Onion Farming: How New Cold Storage in Pampanga is Reducing Losses and Boosting Profits

Revolutionizing Onion Farming: How New Cold Storage in Pampanga is Reducing Losses and Boosting Profits

June 23, 2025
EU-UK Sugar Market Volatility: Weather Woes, Trade Shifts, and Pricing Pressures for 2025/26

EU-UK Sugar Market Volatility: Weather Woes, Trade Shifts, and Pricing Pressures for 2025/26

June 22, 2025
Top 10 Nutrient-Rich Leafy Greens to Boost Your Farm’s Productivity and Profitability

Top 10 Nutrient-Rich Leafy Greens to Boost Your Farm’s Productivity and Profitability

June 22, 2025
Kazakhstan’s Sugar Dilemma: Record Beet Harvest Meets Crumbling Processing Infrastructure

Kazakhstan’s Sugar Dilemma: Record Beet Harvest Meets Crumbling Processing Infrastructure

June 21, 2025
Breaking Ground: Hybrid Sugar Beet Seeds Cultivated in Ipatovsky District – A Step Toward Agricultural Self-Sufficiency

Breaking Ground: Hybrid Sugar Beet Seeds Cultivated in Ipatovsky District – A Step Toward Agricultural Self-Sufficiency

June 21, 2025
Spring Frosts Wipe Out 12% of Sugar Beet Crops – Is Fruit Production Next?

Spring Frosts Wipe Out 12% of Sugar Beet Crops – Is Fruit Production Next?

June 21, 2025
Global Lemon Production Surges in 2025: Mexico Leads, Spain & Emerging Markets Rise

Global Lemon Production Surges in 2025: Mexico Leads, Spain & Emerging Markets Rise

June 20, 2025
Syngenta Dominates Early Piel-de-Sapo Melon Market with 50% Share: How Innovation and Disease Resistance Are Shaping the Future

Syngenta Dominates Early Piel-de-Sapo Melon Market with 50% Share: How Innovation and Disease Resistance Are Shaping the Future

June 20, 2025
Pepper Market Turbulence: Oversupply Sparks Price Crash in the Netherlands – What’s Next?

Pepper Market Turbulence: Oversupply Sparks Price Crash in the Netherlands – What’s Next?

June 19, 2025
  • Company
Wednesday, June 25, 2025
Vegetables News
  • NEWS
    Peruvian Asparagus in Europe: Seizing the Seasonal Window for Export Growth

    Peruvian Asparagus in Europe: Seizing the Seasonal Window for Export Growth

    Brazil’s Fruit Export Paradox: Why the World’s Third-Largest Producer Struggles to Dominate Global Markets

    Brazil’s Fruit Export Paradox: Why the World’s Third-Largest Producer Struggles to Dominate Global Markets

    Price Volatility in Vegetable Markets: Analyzing Trends in Muhana Mandi and Their Impact on Farmers

    Price Volatility in Vegetable Markets: Analyzing Trends in Muhana Mandi and Their Impact on Farmers

    Revolutionizing Onion Farming: How New Cold Storage in Pampanga is Reducing Losses and Boosting Profits

    Revolutionizing Onion Farming: How New Cold Storage in Pampanga is Reducing Losses and Boosting Profits

    EU-UK Sugar Market Volatility: Weather Woes, Trade Shifts, and Pricing Pressures for 2025/26

    EU-UK Sugar Market Volatility: Weather Woes, Trade Shifts, and Pricing Pressures for 2025/26

    Top 10 Nutrient-Rich Leafy Greens to Boost Your Farm’s Productivity and Profitability

    Top 10 Nutrient-Rich Leafy Greens to Boost Your Farm’s Productivity and Profitability

    Kazakhstan’s Sugar Dilemma: Record Beet Harvest Meets Crumbling Processing Infrastructure

    Kazakhstan’s Sugar Dilemma: Record Beet Harvest Meets Crumbling Processing Infrastructure

    Breaking Ground: Hybrid Sugar Beet Seeds Cultivated in Ipatovsky District – A Step Toward Agricultural Self-Sufficiency

    Breaking Ground: Hybrid Sugar Beet Seeds Cultivated in Ipatovsky District – A Step Toward Agricultural Self-Sufficiency

    Spring Frosts Wipe Out 12% of Sugar Beet Crops – Is Fruit Production Next?

    Spring Frosts Wipe Out 12% of Sugar Beet Crops – Is Fruit Production Next?

  • AGROTECHNOLOGY
    • All
    • Climate
    • Crop protection
    • digital (smart)
    • dron
    • Fertilizer
    • Research
    • weed
    Innovative Cherry Farming in Vorarlberg: High Yields, Smart Techniques, and Sustainable Practices

    Innovative Cherry Farming in Vorarlberg: High Yields, Smart Techniques, and Sustainable Practices

    Unseasonal Rains Wreak Havoc on Gujarat’s Onion Crop: Price Surge and Farmer Distress Loom

    Unseasonal Rains Wreak Havoc on Gujarat’s Onion Crop: Price Surge and Farmer Distress Loom

    High-Residue Cover Cropping: A Viable Alternative to Plastic Mulch for Organic Vegetable Growers?

    High-Residue Cover Cropping: A Viable Alternative to Plastic Mulch for Organic Vegetable Growers?

    EU Pesticide Sales Hit 12-Year Low in 2023: What It Means for Modern Agriculture

    EU Pesticide Sales Hit 12-Year Low in 2023: What It Means for Modern Agriculture

    Why Do Grocers Cover Potatoes at Night? The Science Behind This Curious Practice

    Why Do Grocers Cover Potatoes at Night? The Science Behind This Curious Practice

    Record-Early Potato Planting in 2024: Will It Lead to Higher Yields?

    Record-Early Potato Planting in 2024: Will It Lead to Higher Yields?

    Boosting Farm Efficiency: How Lean Technologies Are Transforming Agriculture in Altai

    Boosting Farm Efficiency: How Lean Technologies Are Transforming Agriculture in Altai

    Rising Temperatures Threaten Vegetable Farming in Maharashtra: Challenges and Solutions for Farmers

    Rising Temperatures Threaten Vegetable Farming in Maharashtra: Challenges and Solutions for Farmers

    Boosting Welsh Agriculture: How Organic School Meals Could Transform Farming by 2030

    Boosting Welsh Agriculture: How Organic School Meals Could Transform Farming by 2030

  • IRRIGATION
  • SEED PRODUCTION
    • Vegetables
    • Fruit
  • PROCESSING
No Result
View All Result
  • NEWS
    Peruvian Asparagus in Europe: Seizing the Seasonal Window for Export Growth

    Peruvian Asparagus in Europe: Seizing the Seasonal Window for Export Growth

    Brazil’s Fruit Export Paradox: Why the World’s Third-Largest Producer Struggles to Dominate Global Markets

    Brazil’s Fruit Export Paradox: Why the World’s Third-Largest Producer Struggles to Dominate Global Markets

    Price Volatility in Vegetable Markets: Analyzing Trends in Muhana Mandi and Their Impact on Farmers

    Price Volatility in Vegetable Markets: Analyzing Trends in Muhana Mandi and Their Impact on Farmers

    Revolutionizing Onion Farming: How New Cold Storage in Pampanga is Reducing Losses and Boosting Profits

    Revolutionizing Onion Farming: How New Cold Storage in Pampanga is Reducing Losses and Boosting Profits

    EU-UK Sugar Market Volatility: Weather Woes, Trade Shifts, and Pricing Pressures for 2025/26

    EU-UK Sugar Market Volatility: Weather Woes, Trade Shifts, and Pricing Pressures for 2025/26

    Top 10 Nutrient-Rich Leafy Greens to Boost Your Farm’s Productivity and Profitability

    Top 10 Nutrient-Rich Leafy Greens to Boost Your Farm’s Productivity and Profitability

    Kazakhstan’s Sugar Dilemma: Record Beet Harvest Meets Crumbling Processing Infrastructure

    Kazakhstan’s Sugar Dilemma: Record Beet Harvest Meets Crumbling Processing Infrastructure

    Breaking Ground: Hybrid Sugar Beet Seeds Cultivated in Ipatovsky District – A Step Toward Agricultural Self-Sufficiency

    Breaking Ground: Hybrid Sugar Beet Seeds Cultivated in Ipatovsky District – A Step Toward Agricultural Self-Sufficiency

    Spring Frosts Wipe Out 12% of Sugar Beet Crops – Is Fruit Production Next?

    Spring Frosts Wipe Out 12% of Sugar Beet Crops – Is Fruit Production Next?

  • AGROTECHNOLOGY
    • All
    • Climate
    • Crop protection
    • digital (smart)
    • dron
    • Fertilizer
    • Research
    • weed
    Innovative Cherry Farming in Vorarlberg: High Yields, Smart Techniques, and Sustainable Practices

    Innovative Cherry Farming in Vorarlberg: High Yields, Smart Techniques, and Sustainable Practices

    Unseasonal Rains Wreak Havoc on Gujarat’s Onion Crop: Price Surge and Farmer Distress Loom

    Unseasonal Rains Wreak Havoc on Gujarat’s Onion Crop: Price Surge and Farmer Distress Loom

    High-Residue Cover Cropping: A Viable Alternative to Plastic Mulch for Organic Vegetable Growers?

    High-Residue Cover Cropping: A Viable Alternative to Plastic Mulch for Organic Vegetable Growers?

    EU Pesticide Sales Hit 12-Year Low in 2023: What It Means for Modern Agriculture

    EU Pesticide Sales Hit 12-Year Low in 2023: What It Means for Modern Agriculture

    Why Do Grocers Cover Potatoes at Night? The Science Behind This Curious Practice

    Why Do Grocers Cover Potatoes at Night? The Science Behind This Curious Practice

    Record-Early Potato Planting in 2024: Will It Lead to Higher Yields?

    Record-Early Potato Planting in 2024: Will It Lead to Higher Yields?

    Boosting Farm Efficiency: How Lean Technologies Are Transforming Agriculture in Altai

    Boosting Farm Efficiency: How Lean Technologies Are Transforming Agriculture in Altai

    Rising Temperatures Threaten Vegetable Farming in Maharashtra: Challenges and Solutions for Farmers

    Rising Temperatures Threaten Vegetable Farming in Maharashtra: Challenges and Solutions for Farmers

    Boosting Welsh Agriculture: How Organic School Meals Could Transform Farming by 2030

    Boosting Welsh Agriculture: How Organic School Meals Could Transform Farming by 2030

  • IRRIGATION
  • SEED PRODUCTION
    • Vegetables
    • Fruit
  • PROCESSING
No Result
View All Result
Vegetables News
Home AGROTECHNOLOGY Crop protection

Development and validation of a multiplex real-time RT-PCR test for the screening of tomato (Solanum lycopersicum) for the detection of pepino mosaic virus

by Maria Yerokhova
August 28, 2024
in Crop protection
0
Development and validation of a multiplex real-time RT-PCR test for the screening of tomato (Solanum lycopersicum) for the detection of pepino mosaic virus

Pepino mosaic virus (PepMV) is known to cause a wide range of symptoms in cultivated tomato plants, damaging fruits and crops. Currently, five genotypes of PepMV are known (US1, CH2, EU, LP and PES), which can all infect tomato plants. Tomato seed is a proven pathway for PepMV, and the virus is known to easily spread mechanically during crop handling. A multiplex real-time reverse transcriptase-polymerase chain reaction (RT-PCR) test was developed and validated for the specific identification of PepMV isolates from tomato seeds. This test is designed to be used for diagnostics and seed health tests as a pre-screen to distinguish negative PepMV samples from suspect samples or as a confirmation test of a positive ELISA or bioassay test result. The multiplex real-time RT-PCR test consists of two PepMV-specific real-time RT-PCR tests and a Bacopa chlorosis virus real-time RT-PCR test as an internal amplification control. The analytical specificity of the developed test was evaluated by in vitro and in silico analyses. The analytical sensitivity was evaluated by creating three dilution series of naturally PepMV-infected leaf tissue in healthy tomato seed extracts and the diagnostic specificity and sensitivity were validated on a collection of seed lots.

Photo: EPPO (2024) EPPO Global Database. https://gd.eppo.int 

Reference: Berendsen, S.M.H., Thomas, L.A., Woudenberg, J.H.C., Speksnijders, Y., Jonkers, W., Pannu, S. et al. (2024) Development and validation of a multiplex real-time RT-PCR test for the screening of tomato (Solanum lycopersicum) for the detection of pepino mosaic virus. EPPO Bulletin, 54, 243–251. Available from: https://doi.org/10.1111/epp.13027

Tags: Pepino Mosaic VirusSolanum lycopersicum
Previous Post

Navigating the Future: How Chile’s Kiwi Industry Must Adapt in a Competitive Global Market

Next Post

Record Prices Set the Stage for Poland’s Apple Season: What Farmers Need to Know

Next Post
Record Prices Set the Stage for Poland’s Apple Season: What Farmers Need to Know

Record Prices Set the Stage for Poland's Apple Season: What Farmers Need to Know

  • Home
  • Privacy Policy
  • Privacy Policy of vegetables.news app

© 2020-2024 VEGETABLES NEWS

No Result
View All Result
  • NEWS
  • AGROTECHNOLOGY
  • IRRIGATION
  • SEED PRODUCTION
    • Vegetables
    • Fruit
  • PROCESSING

© 2020-2024 VEGETABLES NEWS